鈣通量測定法是藥品發(fā)現(xiàn)中篩選G蛋白偶聯(lián)受體（GPCR）的方法。 Screen Quest Rhod-4 NW鈣含量測定試劑盒提供了一種基于熒光的均相測定，用于檢測細(xì)胞內(nèi)鈣的遷移。Rhod-4是可用于HTS篩選的亮的紅色鈣指示劑。一旦進(jìn)入細(xì)胞內(nèi)，Rhod-4 的親脂性封閉基團(tuán)就會被非特異性細(xì)胞酯酶裂解，產(chǎn)生帶負(fù)電荷的熒光染料，并留在細(xì)胞內(nèi)部，并且與鈣結(jié)合后其熒光會大大增強(qiáng)。當(dāng)細(xì)胞被篩選化合物刺激時(shí)，受體信號釋放細(xì)胞內(nèi)鈣，這大大增加了Rhod-4的熒光。 Rhod-4 具有長波長，高靈敏度和大于250倍的熒光增強(qiáng)特性（當(dāng)與鈣形成復(fù)合物時(shí)），使其成為測量細(xì)胞鈣的理想指標(biāo)。此Screen Quest Rhod-4 NW鈣測定試劑盒提供了一種優(yōu)化的測定方法，用于檢測G蛋白偶聯(lián)受體（GPCR）和鈣通道。該測定可以用96孔或384孔微孔板進(jìn)行，并易于適應(yīng)自動化。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest 免洗Rhod-4鈣檢測試劑盒。

參考文獻(xiàn)

Fluorescence absorbance inner-filter decomposition: the role of emission shape on estimates of free Ca(2+) using Rhod-2
Authors: Territo PR, Heil J, Bose S, Evans FJ, Balaban RS.
Journal: Appl Spectrosc (2007): 138

Kinetic characterization of novel NR2B antagonists using fluorescence detection of calcium flux
Authors: Bednar B, Cunningham ME, Kiss L, Cheng G, McCauley JA, Liverton NJ, Koblan KS.
Journal: J Neurosci Methods (2004): 247

Novel fluo-4 analogs for fluorescent calcium measurements
Authors: Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR.
Journal: Cell Calcium (2004): 509

Protein kinase C and myocardial calcium handling during ischemia and reperfusion: lessons learned using Rhod-2 spectrofluorometry
Authors: Stamm C, del Nido PJ.
Journal: Thorac Cardiovasc Surg (2004): 127

Cytosolic calcium in the ischemic rabbit heart: assessment by pH- and temperature-adjusted rhod-2 spectrofluorometry
Authors: Stamm C, Friehs I, Choi YH, Zurakowski D, McGowan FX, del Nido PJ.
Journal: Cardiovasc Res (2003): 695