Screen Quest 熒光ELISA cAMP分析試劑盒提供了一種優(yōu)化的分析方法，用于檢測G蛋白偶聯(lián)受體系統(tǒng)中腺苷酸環(huán)化酶的。該測定基于HRP標記的cAMP與未標記的cAMP之間固定數(shù)目的抗體結合位點的競爭。通過未標記的游離cAMP將HRP-cAMP從HRP-cAMP /抗cAMP抗體復合物中置換出來。在不存在cAMP的情況下，HRP-cAMP偶聯(lián)物僅與抗cAMP抗體結合。然而，測試樣品中未標記的游離cAMP與HRP-cAMP抗體偶聯(lián)物競爭抗cAMP抗體，因此抑制了HRP-cAMP與抗cAMP抗體的結合。我們的Screen Quest 熒光cAMP分析試劑盒提供了檢測腺苷酸環(huán)化酶活性的靈敏方法。與其他商用ELISA cAMP分析試劑盒相比，它繁瑣的乙?；襟E。該試劑盒使用Amplite Red作為熒光HRP底物來定量HRP活性。形成的熒光產(chǎn)物與HRP-cAMP偶聯(lián)物的活性成比例。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest cAMP檢測試劑盒。

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